Vitamin D 25-Hydroxy: Total D plus D2, D3

Vitamin D 25-Hydroxy: Total D plus D2, D3


Sample Test Result
SKU: Vitamin D 25-Hydroxyvitamin D (D2, D3) Category:

QuestAssureDâ„¢ 25-Hydroxyvitamin D (D2, D3) – Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 – the endogenous form of the vitamin and 25-OH D2 – the analog form used to treat 25-OH Vitamin D3 deficiency.

Clinical Focus
  • Vitamin D, 25-OH, Total
  • Vitamin D, 25-OH, D3
  • Vitamin D, 25-OH, D2

Patient Preparation

Fasting preferred, but not required


Chromatography/Mass Spectrometry

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established

Alternative Name(s)

Vitamin D2, 25-hydroxy,25-Hydroxyergocalciferol (25OHD2),Vitamin D3, 25-hydroxy,D2 Vitamin,25-Hydroxyvitamin D3,25-OH-D3,25-Hydroxycholecalciferol (25OHD3),25-OH-D2,D3 Vitamin,Vit D, LC/MS/MS,25-Hydroxyvitamin Quest code 92888

Please be advised, that our services are strictly self pay and are not eligible for submission as a claim to your health insurance provider. However, you can submit the receipt for reimbursement to many Flexible Spending and Health Savings Accounts for reimbursement.

My Account