Nicotine & Expanded Metabolites (urine)

Nicotine & Expanded Metabolites (urine)


SKU: Nicotine & Expanded Metabolites (urine) Category:

CPT Code(s)

80323 (HCPCS: G0480)


Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Reference Range(s)

Active Tobacco User
Nicotine <17 200-700
Cotinine <20 300-1300
3-OH-Cotinine <50 3000-25000
Nornicotine <2 30-900
Norcotinine <2 Not established
Anabasine <2 10-500


Clinical Significance

This assay is used for the detection of nicotine and several metabolites and the related alkaloid anabasine in urine to determine the tobacco exposure status of the individual. Nicotine has a short half-life of approximately sixty minutes; Its presence may indicate recent tobacco exposure. The half-life of the various nicotine metabolites is longer and provides a larger window of detection for tobacco exposure. The presence of anabasine is indicative of tobacco exposure (smoked or smokeless) versus the use of a nicotine replacement patch product. Anabasine is a related alkaloid found only in the tobacco plant. Individuals exposed to passive tobacco smoke (environmental or second-hand) can have detectable concentrations of nicotine and its metabolites depending on the type of exposure. The ranges of concentrations related to passive exposure are not well established but normally are less than those attributed to active tobacco users.

Please be advised, that our services are strictly self pay and are not eligible for submission as a claim to your health insurance provider. However, you can submit the receipt for reimbursement to many Flexible Spending and Health Savings Accounts for reimbursement.

Alternative Name(s)


This is a non-fasting, urine test. Quest test code 90982


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