In humans, steroid hormones are produced by the adrenal glands and the gonads. The formation of pregnenolone from cholesterol is the first step in steroidogenesis and is mediated by the proteolytic enzyme, single cholesterol side-chain cleavage enzyme . Preparation: No special preparation is needed. Fasting is not required for this blood test. In general, being well hydrated (drinking plenty of water before the blood draw) will usually make your veins somewhat easier to locate and access for the blood draw. Expect results in 6-8 days. Quest test code 31493 Please be advised, that our services are strictly self pay and are not eligible for submission as a claim to your health insurance provider. However, you can submit the receipt for reimbursement to many Flexible Spending and Health Savings Accounts for reimbursement.